亚洲成a人片在线观看www-亚洲成a人片在线观看久-亚洲成a人片在线观看天堂无码-亚洲成a人片在线观看无码-亚洲成a人片在线观看无码专区-亚洲成a人片在线观看尤物

明星產(chǎn)品 Star Products
聯(lián)系方式
深圳艾普諾生物醫(yī)療科技有限公司

地址:深圳市光明區(qū)高新技術產(chǎn)業(yè)園區(qū)邦凱路(光源五路)9號寶新科技園 4號樓10層1006房

電話:0755-89358349

電話:18676399004

QQ:287749897

郵箱: service@aipunuobio.com

明星產(chǎn)品

您當前的位置:首頁 > 明星產(chǎn)品
GAPDH Mouse mAb (High Dilution)
產(chǎn)品貨號:AC033
產(chǎn)品品牌:ABclonal
產(chǎn)品分類:抗體
產(chǎn)品規(guī)格:50ul/100ul/200ul
產(chǎn)品價格:

產(chǎn)品介紹

背景

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.
Note:Dilution method
Put 5ul AC033 into 45 ul buffer(with 50% glycerol), diluted 1:10,000 for using. The diluted antibody can be stored at -20℃ without aliquot.

免疫原信息

應用

反應物種Human, Mouse, Rat
驗證應用WBIHCIF
用戶驗證的應用

WB (Mus musculus , Homo sapiens , Rattus norvegicus , Schisandra chinensis)

IP (Mus musculus)

IF (Mus musculus , Homo sapiens)

DB (Homo sapiens)

推薦稀釋比&實驗步驟
  • WB   1:50000 - 1:200000  實驗步驟 ]
  • IHC   1:50 - 1:200  實驗步驟 ]
  • IF   1:50 - 1:200  實驗步驟 ]
抗體稀釋方法Put 5ul AC033 into 45 ul buffer(with 50% glycerol), diluted 1:10,000 for using. The diluted antibody can be stored at -20℃ without aliquot.
理論分子量31kDa/36kDa
實際分子量36KDa
存儲緩沖液Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
實驗方法Western blotting    
陽性樣本HeLa,HepG2,293T,Mouse liver,Mouse kidney,Mouse spleen,Rat liver,Rat kidney
細胞定位Cytoplasm,Membrane,Nucleus,cytoskeleton,cytosol,perinuclear region
純化方式Affinity purification

GAPDH Mouse mAb (High Dilution) 檢測圖片

ABclonal:Western blot - GAPDH Mouse mAb (High Dilution) (AC033) }

Western blot - GAPDH Mouse mAb (High Dilution) (AC033)

Western blot analysis of extracts of various cell lines, using GAPDH Monoclonal Antibody (AC033) at 1:20000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - GAPDH Mouse mAb (High Dilution) (AC033) }

Western blot - GAPDH Mouse mAb (High Dilution) (AC033)

Western blot analysis of extracts of HeLa cells, using GAPDH antibody (AC033) at 1:10000-1:2560000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - GAPDH Mouse mAb (High Dilution) (AC033) }

Immunohistochemistry - GAPDH Mouse mAb (High Dilution) (AC033)

Immunohistochemistry of paraffin-embedded Human esophageal using GAPDH Mouse mAb (AC033) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - GAPDH Mouse mAb (High Dilution) (AC033) }

Immunofluorescence - GAPDH Mouse mAb (High Dilution) (AC033)

Immunofluorescence analysis of C6 cells using GAPDH Mouse mAb (AC033) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GAPDH Mouse mAb (High Dilution) (AC033) }

Immunofluorescence - GAPDH Mouse mAb (High Dilution) (AC033)

Immunofluorescence analysis of NIH-3T3 cells using GAPDH Mouse mAb (AC033) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GAPDH Mouse mAb (High Dilution) (AC033) }

Immunofluorescence - GAPDH Mouse mAb (High Dilution) (AC033)

Immunofluorescence analysis of U-2 OS cells using GAPDH Mouse mAb (AC033) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.


主站蜘蛛池模板: 真实的国产乱xxxx在线播放 | 国产精品日韩在线观看 | 国产精品福利一区二区久久 | 欧美成人免费一区在线播放 | 亚洲人成在线免费观看 | 四虎精品视频 | 国产亚洲精品激情一区二区三区 | 99国产在线观看 | 尹人成人| 狠狠色狠狠色综合久久第一次 | 日本免费一区二区三区毛片 | 亚洲性另类| 国产图片亚洲精品一区 | 成年性视频bbixx | 日本伊人网| 中文字幕在线观看一区二区 | 久久精品国产一区二区三区肥胖 | 色综合色狠狠天天综合色hd | 欧美一级艳片爽快片 | 老色鬼a∨在线视频在线观看 | 一级毛片视屏 | 欧美日韩国产成人高清视频 | 欧美成人黄色网 | 色网址在线观看 | 亚洲欧美另类在线视频 | 全午夜免费一级毛片 | 91国视频在线观看 | 国产伦精品一区二区三区免 | 欧美日韩中文国产一区 | 欧美日韩一区二区亚洲 | 97视频hd| 久操视频网 | 久久影视在线观看 | 国产精品成人免费综合 | 日韩精品欧美一区二区三区 | 亚洲在线视频 | 国产欧美日韩一区二区三区视频 | 国产在线播放一区二区 | 国产九九在线观看播放 | 青青青国产观看免费视频 | 亚洲午夜久久久精品影院视色 |